From 2 November 2020, the autumn semester 2020 will take place online. Exceptions: Courses that can only be carried out with on-site presence.
Please note the information provided by the lecturers via e-mail.

Rudolf Glockshuber: Catalogue data in Autumn Semester 2016

Name Prof. Dr. Rudolf Glockshuber
FieldMolekularbiologie
Address
Inst. f. Molekularbiol.u.Biophysik
ETH Zürich, HPK E 17
Otto-Stern-Weg 5
8093 Zürich
SWITZERLAND
Telephone+41 44 633 68 19
Fax+41 44 633 10 36
E-mailrudi@mol.biol.ethz.ch
DepartmentBiology
RelationshipFull Professor

NumberTitleECTSHoursLecturers
551-0013-AALBiochemistry
Enrolment ONLY for MSc students with a decree declaring this course unit as an additional admission requirement.

Any other students (e.g. incoming exchange students, doctoral students) CANNOT enrol for this course unit.
2 credits4RR. Glockshuber
AbstractThe lecture is a basic introductory course on the molecular principles of biology for students who need to pass this course for admission to their MSc curriculum.
ObjectiveThe goal of this course is to give the students a basic understanding of the molecules that build a cell and make it function, and the basic principles of metabolism and molecular genetics
ContentThe course content is based on the following chapters of the textbook Biochemistry (Berg, Tymoczko, Stryer, 7th edition, 2012, Freeman & Co, New York)

Chapter 1: The molecular design of life
Chapter 2: Protein composition and structure
Chapter 3: Exploring proteins and proteomes
Chapter 4: DNA, RNA and the flow of information
Chapter 5: Exploring Genes and Genomes
Chapter 7: Hemoglobin
Chapter 8: Enzymes and the basic concepts of catalysis
Chapter 11: Carbohydrates
Chapter 12: Lipids and cell membranes
Chapter 15: Metabolism: Basic concepts and design
LiteratureBiochemistry (Berg, Tymoczko, Stryer, 7th edition, 2012, Freeman & Co, New York)
551-0015-00LBiology I2 credits2VR. Glockshuber, E. Hafen
AbstractThe lecture Biology I, together with the lecture Biology II in the following summer semester, is a basic, introductory course into Biology for Students of Materials Sciences and other students with biology as subsidiary subject.
ObjectiveThe goal of this course is to give the students a basic understanding of the molecules that build a cell and make it function, and the basic principles of metabolism and molecular genetics.
ContentDie folgenden Kapitelnummern beziehen sich auf das der Vorlesung zugrundeliegende Lehrbuch "Biology" (Campbell & Rees, 10th edition, 2015)
Kapitel 1-4 des Lehrbuchs werden als Grundwissen vorausgesetzt

1. Aufbau der Zelle

Kapitel 5: Struktur und Funktion biologischer Makromoleküle
Kapitel 6: Eine Tour durch die Zelle
Kaptiel 7: Membranstruktur und-funktion
Kapitel 8: Einführung in den Stoffwechsel
Kapitel 9: Zelluläre Atmung und Speicherung chemischer Energie
Kapitel 10: Photosynthese
Kapitel 12: Der Zellzyklus
Kapitel 17: Vom Gen zum Protein

2. Allgemeine Genetik

Kapitel 13: Meiose und Reproduktionszyklen
Kapitel 14: Mendel'sche Genetik
Kapitel 15: Die chromosomale Basis der Vererbung
Kapitel 16: Die molekulare Grundlage der Vererbung
Kapitel 18: Genetik von Bakterien und Viren
Kapitel 46: Tierische Reproduktion

Grundlagen des Stoffwechsels und eines Überblicks über molekulare Genetik
Lecture notesDer Vorlesungsstoff ist sehr nahe am Lehrbuch gehalten, Skripte werden ggf. durch die Dozenten zur Verfügung gestellt.
LiteratureDas folgende Lehrbuch ist Grundlage für die Vorlesungen Biologie I und II:

„Biology“, Campbell and Rees, 10th Edition, 2015, Pearson/Benjamin Cummings, ISBN 978-3-8632-6725-4
Prerequisites / NoticeZur Vorlesung Biologie I gibt es während der Prüfungssessionen eine einstündige, schriftliche Prüfung. Die Vorlesung Biologie II wird separat geprüft.
551-0307-00LMolecular and Structural Biology I: Protein Structure and Function Information
D-BIOL BSc students are obliged to take part I and part II (next semester) as a two-semester course
3 credits2VR. Glockshuber, K. Locher, E. Weber-Ban
AbstractBiophysics of protein folding, membrane proteins and biophysics of membranes, enzymatic catalysis, catalytic RNA and RNAi, current topics in protein biophysics and structural biology.
ObjectiveUnderstanding of structure-function relationships in proteins and in protein folding, detailed understanding of biophysics and physical methods as well as modern methods for protein purification and microanalytics.
Lecture notesScripts on the individual topics can be found under http://www.mol.biol.ethz.ch/teaching.
LiteratureBasics:
- Creighton, T.E., Proteins, Freeman, (1993)
- Fersht, A., Enzyme, Structure and Mechanism in Protein Science (1999), Freeman.
- Berg, Tymoczko, Stryer: Biochemistry (5th edition), Freeman (2001).

Current topics: References will be given during the lectures.

.
551-0438-00LProtein Folding, Assembly and Degradation Restricted registration - show details
Number of participants limited to 6.
6 credits7GR. Glockshuber, E. Weber-Ban
AbstractStudents will carry out defined research projects related to the current research topics of the groups of Prof. Glockshuber and Prof. Weber-Ban. The topics include mechanistic studies on the assembly of adhesive pili from pathogenic bacteria, disulfide bond formation in the bacterial periplasm, ATP-dependent chaperone-protease complexes and formation of amyloid deposits in Alzheimer's disese.
ObjectiveThe course should enable the students to understand and apply biophysical methods, in particular kinetic and spectroscopic methods, to unravel the mechanism of complex reactions of biological macromolecules and assemblies in a quantitative manner.
ContentThe students will be tutored in their experimental work by doctoral or postdoctoral students from the Glockshuber or Weber-Ban group. In addition, the course includes specific lectures that provide the theoretical background for the experimental work, as well as excercises on the numeric evaluation of biophysical data, and literature work.

Participation in one of the following projects will be possible:

Projects of the Glockshuber group:
- Purification, biophysical characterization and structure determiation of enzymes required for disulfide bond formation in the periplasm of Gram-negative bacteria.
- Mechanistic studies on the assembly of type 1 pili from pathogenic Escherichia coli strains. In vitro reconstitution of pilus assembly from all purified components. Characterization of folding, stability and assembly behaviour of individual pilus subunits.
- Identification of intermediates in the aggregation of the human Abeta peptide

Experimental work on these projects involves
- Molecular cloning, recombinant protein production in E. coli and protein purification
- Protein crystallization
- Thermodynamic and kinetic characterization of conformational changes in proteins and protein-ligand interactions by fluorescence and circular dischoism spectroscopy
- Analysis of rapid reactions by stopped-flow fluorescence
- Negative-stain electron microscopy
- Light scattering



Projects of the Weber-Ban group:

- Generation and purification of site-directed variants of the E. coli ClpA/P protease and chaperone-proteasome complexes from other organisms, their biophysical characterization, including rapid kinetics by stopped-flow methods, ATPase activity measurtements, negative-stain electron microscopy and light scattering
Lecture notesNo script
LiteratureLiterature related to the individual projects will be provided on the first day of the course.
Prerequisites / NoticeAttendance of the concept course "Biomolecular Structure and Mechanism I: Protein Structure and Function" (551-0307-00L) in the autumn semester is highly recommended for acquiring the theoretical background to this block course.
551-1323-AALFundamentals of Biology II: Biochemistry and Molecular Biology
Enrolment ONLY for MSc students with a decree declaring this course unit as an additional admission requirement.

Any other students (e.g. incoming exchange students, doctoral students) CANNOT enrol for this course unit.
4 credits11RK. Locher, N. Ban, R. Glockshuber, E. Weber-Ban
AbstractThe course provides an introduction to Biochemistry / Molecular Biology with some emphasis on chemical and biophysical aspects.
ObjectiveTopics include the structure-function relationship of proteins / nucleic acids, protein folding, enzymatic catalysis, cellular pathways involved in bioenergetics and the biosynthesis and breakdown of amino acids, glycans, nucleotides, fatty acids and phospholipids, and steroids. There will also be a discussion of DNA replication and repair, transcription, and translation.
Lecture notesnone
Literature"Biochemistry",
Berg/Tymoczko/Stryer, 8th edition, Palgrave Macmillan, International edition
551-1323-00LFundamentals of Biology II: Biochemistry and Molecular Biology Information 4 credits4VK. Locher, N. Ban, R. Glockshuber, E. Weber-Ban
AbstractThe course provides an introduction to Biochemistry / Molecular Biology with some emphasis on chemical and biophysical aspects.
ObjectiveTopics include the structure-function
relationship of proteins / nucleic acids, protein folding, enzymatic catalysis, cellular pathways involved in bioenergetics and the biosynthesis and breakdown of amino acids, glycans, nucleotides, fatty acids and phospholipids, and steroids. There will also be a discussion of DNA replication and repair, transcription, and translation.
Lecture notesnone
Literaturemandatory: "Biochemistry",
Autoren: Berg/Tymoczko/Stryer, 8th edition, Palgrave Macmillan, International edition (the English version will be preordered at the Polybuchhandlung)
Prerequisites / NoticeSome of the lectures are given in the English language.
551-1411-00LMolecular and Structural Biology III: Current Topics Restricted registration - show details
The course will only take place with a minimum of 6 participants.
2 credits1VK. Locher, F. Allain, N. Ban, R. Glockshuber, M. Pilhofer, E. Weber-Ban
AbstractThe course discusses current topics and cutting edge research in the structural, molecular, and biochemical study of cellular macromolecules. Student participation is an essential component of the course and will contribute to the exam grade
ObjectiveThe goal is to discuss cutting edge research in the structural, molecular, and biochemical study of cellular macromolecules. Students will also have an opportunity to present and discuss recent breakthroughs relevant to the research fields presented by the faculty teaching the course (see http://www.mol.biol.ethz.ch/research.html for details on the topics).
LiteratureWill be distributed by the instructors
551-1619-00LStructural Biology1 credit1KR. Glockshuber, F. Allain, N. Ban, K. Locher, E. Weber-Ban, G. Wider, K. Wüthrich
AbstractThe course consists of a series of research seminars on Structural Biology, Biochemistry and Biophysics, given by both scientists of the National Center of Competence in Research (NCCR) in Structural Biology and external speakers. Information on the individual seminars is provided on the following websites:
http://www.structuralbiology.uzh.ch/educ002.asp
http://www.biol.ethz.ch/dbiol-cal/index
ObjectiveThe goal of this course is to provide doctoral and postdoctoral students with a broad overview on the most recent developments in biochemistry, structural biology and biophysics.