Paola Picotti: Catalogue data in Spring Semester 2019

Name Prof. Dr. Paola Picotti
FieldProtein Network Biology
Inst. f. Molekulare Systembiologie
ETH Zürich, HPM H 46
Otto-Stern-Weg 3
8093 Zürich
Telephone+41 44 633 25 58
Fax+41 44 633 12 98
RelationshipAssociate Professor

551-0352-00LProtein Analysis by Mass Spectrometry Restricted registration - show details
Number of participants limited to 12.

The enrolment is done by the D-BIOL study administration.
6 credits7GL. Gillet, B. Collins, P. Picotti
AbstractProtein Analysis by Mass Spectrometry
The following topics will be covered: basics of biological mass spectrometry, including instrumentation, data collection and data analysis; applications to protein identification and characterization; sample preparation methods; proteomics strategies; and quantitative analysis.
ObjectiveHow to prepare a protein sample for MS analysis (trypsin digestion, C18 clean-up)
Principles of data acquisition LC-MS (QTOF and/or Ion Trap instruments)
Perform qualitative proteomic analysis (protein identification with Mascot and/or Sequest Softwares)
Perform quantitative proteomic analysis (label-free and labeled analyses)
Analyze/interpret the data to find up/down regulated proteins