Yaakov Benenson: Catalogue data in Spring Semester 2020

Name Prof. Dr. Yaakov Benenson
FieldSynthetic Biology
Professur f. Synthetische Biologie
ETH Zürich, BSA N 860
Mattenstr. 26
4058 Basel
Telephone+41 61 387 33 38
DepartmentBiosystems Science and Engineering
RelationshipAssociate Professor

636-0205-00LLab Course: Mammalian Gene Circuits Restricted registration - show details 2 credits5PY. Benenson
AbstractThe students are trained in basic techniques in construction and characterization of synthetic gene circuits in mammalian cells. Experimental circuits are built with both the input and the output conjugated to fluorescent reporters, allowing characterization at the single cell level.
ObjectiveThe objective of the course is to construct a genetic sensor for a molecular regulatory input such as microRNA or a transcription factor and characterize the input/output relationship of this sensor with the help of fluorescent reporters, fluorescent microscopy and fluorescent-activated cell sorting. The emphasis is on single-cell characterization.
ContentThe course will take place over 4 weeks, with 2 days per week spent on lab work. The 4 weeks will be dedicated to the following activities

Week 1: Introduction to the course; supervised construct design and detailed planning. Cloning of the constructs: part 1.
Week 2: Cloning of the constructs, purification and characterization of DNA constructs
Week 3: Cell culture transfection, microscopy and flow cytometry characterization
Week 4: Data analysis and preparation of the final report; possibility to repeat failed experiments.
Lecture notesPreparatory materials will be provided before the start of the course.
LiteratureWill be provided before the course
636-0301-00LCurrent Topics in Biosystems Science and Engineering2 credits1SR. Platt, N. Beerenwinkel, Y. Benenson, K. M. Borgwardt, P. S. Dittrich, M. Fussenegger, A. Hierlemann, D. Iber, M. H. Khammash, D. J. Müller, S. Panke, S. Reddy, T. Schroeder, T. Stadler, J. Stelling, B. Treutlein, C. Uhler
AbstractThis seminar will feature invited lectures about recent advances and developments in systems biology, including topics from biology, bioengineering, and computational biology.
ObjectiveTo provide an overview of current systems biology research.
ContentThe final list of topics will be available at http://www.bsse.ethz.ch/education/.