Ludovic Gillet: Catalogue data in Spring Semester 2021
|Name||Dr. Ludovic Gillet|
Inst. f. Molekulare Systembiologie
ETH Zürich, HPM F 42
|551-0224-00L||Advanced Proteomics |
For master students from the 2nd semester on, also doctoral candidates and post docs.
|4 credits||6G||P. Picotti, L. Gillet, A. Leitner, P. Pedrioli|
|Abstract||Goal of the course is to analyze current and newly emerging technologies and approaches in protein and proteome analysis with regard to their application in biology, biotechnology and medicine.|
Format: Introduction by instructor followed by discussions stimulated by reading assignments and exercises.
|Objective||To discuss current and newly emerging technologies and approaches in protein and proteome analysis with regard to their applications in biology, biotechnology, medicine and systems biology.|
|Content||Block course teaching current methods for the acquisition and processing of proteomic datasets.|
|Prerequisites / Notice||Number of people: Not exceeding 30.|
Students from ETHZ, Uni Zurich and University of Basel
Non-ETH students must register at ETH Zurich as special students http://www.rektorat.ethz.ch/students/admission/auditors/index_EN
|551-0352-00L||Introduction to Mass Spectrometry-Based Proteomics |
Number of participants limited to 12.
The enrolment is done by the D-BIOL study administration.
General safety regulations for all block courses:
-Whenever possible the distance rules have to be respected
-All students have to wear masks throughout the course. Please keep reserve masks ready. Surgical masks (IIR) or medical grade masks (FFP2) without a valve are permitted. Community masks (fabric masks) are not allowed.
-The installation and activation of the Swiss Covid-App is highly encouraged
-Any additional rules for individual courses have to be respected
-Students showing any COVID-19 symptoms are not allowed to enter ETH buildings and have to inform the course responsible
|6 credits||7P||L. Gillet, P. Picotti|
|Abstract||Protein Analysis by Mass Spectrometry|
The following topics will be covered: basics of biological mass spectrometry, including instrumentation, data collection and data analysis; applications to protein identification and characterization; sample preparation methods; proteomics strategies; and quantitative analysis.
|Objective||How to prepare a protein sample for MS analysis (trypsin digestion, C18 clean-up)|
Principles of data acquisition LC-MS (QTOF and/or Ion Trap instruments)
Perform qualitative proteomic analysis (protein identification with Mascot and/or Sequest Softwares)
Perform quantitative proteomic analysis (label-free and labeled analyses)
Analyze/interpret the data to find up/down regulated proteins