Jörn Piel: Catalogue data in Spring Semester 2019
|Name||Prof. Dr. Jörn Piel|
Institut für Mikrobiologie
ETH Zürich, HCI G 431
|Telephone||+41 44 633 07 55|
|551-0110-00L||Fundamentals of Biology II: Microbiology||2 credits||2V||J. Vorholt-Zambelli, W.‑D. Hardt, J. Piel|
|Abstract||Bacterial cell biology, molecular genetics, gene regulation, growth physiology, metabolism (Bacteria and Archaea), natural products, microbial interactions|
|Objective||Basic principles of cell structure, growth physiology, energy metabolism, gene expression and regulation. Biodiversity of Bacteria and Archaea. Phylogeny and evolution.|
|Content||Bacterial cell biology, molecular genetics, gene regulation, growth physiology, metabolism (Bacteria and Archaea), natural products, microbial interactions|
|Literature||Brock, Biology of Microorganisms (Madigan, M.T. and Martinko, J.M., eds.), 14th ed., Pearson Prentice Hall, 2015|
|551-0314-00L||Microbiology (Part II)||3 credits||2V||W.‑D. Hardt, L. Eberl, H.‑M. Fischer, J. Piel, J. Vorholt-Zambelli|
|Abstract||Advanced lecture class providing a broad overview on bacterial cell structure, genetics, metabolism, symbiosis and pathogenesis.|
|Objective||This concept class will be based on common concepts and introduce to the enormous diversity among bacteria and archaea. It will cover the current research on bacterial cell structure, genetics, metabolism, symbiosis and pathogenesis.|
|Content||Advanced class covering the state of the research in bacterial cell structure, genetics, metabolism, symbiosis and pathogenesis.|
|Lecture notes||Updated handouts will be provided during the class.|
|Literature||Current literature references will be provided during the lectures.|
|Prerequisites / Notice||English|
|551-1106-00L||Progress Reports in Microbiology and Immunology|
Students must sign up via secr.micro.biol.ethz.ch
|0 credits||5S||J. Piel, M. Aebi, H.‑M. Fischer, W.‑D. Hardt, A. Oxenius, J. Vorholt-Zambelli|
|Abstract||Presentation and discussion of current research results in the field of Microbiology and Infection Immunology|
|Objective||Precise and transparent presentation of research findings in relation to the current literature, critical discussion of experimental data and their interpretation, development and presentation of future research aims|
|551-1109-00L||Seminars in Microbiology||0 credits||2K||M. Aebi, W.‑D. Hardt, M. Künzler, J. Piel, S. Sunagawa, J. Vorholt-Zambelli|
|Abstract||Seminars by invited speakers covering selected microbiology themes.|
|Objective||Discussion of selected microbiology themes presented by invited speakers.|
|551-1147-00L||Bioactive Natural Products from Bacteria |
Number of participants limited to 7.
The enrolment is done by the D-BIOL study administration.
|6 credits||7G||J. Piel|
|Abstract||Lab course. In small groups projects of relevance to current research questions in the field of bacterial natural product biosynthesis are addressed.|
|Objective||Introduction to relevant subjects of the secondary metabolism of bacteria. Training in practical work in a research laboratory. Scientific writing in form of a research report.|
|Content||Research project on bacteria that produce bioactive natural products (e.g., Streptomycetes, Cyanobacteria, uncultivated bacteria). The techniques used will depend on the project, e.g. PCR, cloning, natural product analysis, precursor feeding studies, enzyme expression and analysis.|
|Literature||Will be provided for each of the projects at the beginning of the course.|
|551-1298-00L||Genetics, Genomics, Bioinformatics||4 credits||2V + 2U||E. Hafen, C. Beyer, B. Christen, U. K. Genick, J. Piel, R. Schlapbach, G. Schwank, S. Sunagawa, K. Weis, A. Wutz|
|Abstract||The course provides the basis of modern genetics, genomics and bioinformatics. A special focus is placed on the use of these tools for the understanding of biological processes in bacteria, model organisms and humans. The unit uses the principle of blended learning consisting of self-study modules in Moodle, tasks and input lectures by experts from the department.|
|Objective||At the end of this course you know the most important genetic tools in different organisms. You can use the essential methods in bioinformatics by using online tools. You know the advantages and disadvantages of various model organisms to understand biological processes. You know the various mutagenesis methods and other tools to disrupt gene function and can discuss their merits and drawbacks. You are aware of the difficulties in choosing a phenotype for selection in a mutagenesis experiment. Finally, you can describe how you would study a specific biological process by choosing a model organism and the appropriate genetic or genomic tools.|
|Content||The appearance and function of an organism (phenotype) is determined by the interplay between its genome (genotype) and the environment: Genotype + environment = phenotype. Understanding these interactions to the point where we can ultimately predict the phenotype from knowledge of the genotype and environmental factors is one oft the great challenges of biology.|
In the course Bio IA you learnt about the composition and function of the genome and how it is inherited. The goal of this course is that you learn how genetic, genomic and bioinformatics methods are used to understand biological processes (the connection between genotype and phenotype).
In the first two weeks you will renew and deepen your knowledge of the basic principles of genetics and genomics in interactive learning modules on the Moodle platform. This is followed by an introduction of the basic tools of bioinformatics. You learn to search for specific genome sequences, to align them and to construct pedigrees of related genes.
After you have mastered the basic principles you will learn how to study biological processes either by inactivating specific genes or by randomly mutagenizing the entire genome. You will be introduced to different model organisms (bacteria, yeast, Drosophila, mouse) and humans.
Conventional genetic methods rely on the alteration of the function of single genes and on the observation of the effect on the organism (phenotype). Based on the observed phenotype one deduces the normal function of the gene. This is a strong simplification since, even if environmental factors are controlled, phenotypes are very rarely controlled by a single gene. It is therefore important to understand the influence of the entire genome in conjunction with environmental factors on a given phenotype (e.g. a human disease). Modern methods in genomics now permit first approaches in this direction. Therefore, the focus of the second part of the unit is on genome-wide association studies. You learn, how the influence of the entire genome on a specific phenotype is detected and what challenges are involved in the analysis and the interpretation of the results. We will examine these methods in model organisms and humans. You will also learn how the genome of cancer cells changes under the constant selection for these cells to survive and how this genome analysis provides new insights into diagnosis and therapy.
This course is based on active learning. Each week consists of a learning unit with clearly defined learning goals. In the first two hours you will learn the basics from texts, videos and questionnaires on the Moodle platform. In the third lecture an expert on the topic of the week (e.g. genetic screens in yeast) from the department will give an input lecture that builds on the basic knowledge that you acquired. In the fourth lecture you will discuss the tests and topics of the week with the expert. During the semester you will have access to assistants and lecturers via the Moodle online forum.
At the beginning of the learning unit you will take a short multiple-choice test on the content of the course. This formative assessment does not count for your final grade but gives you and us a way to determine where you stand also in relation to your fellow students. A similar formative assessment test will be given at the end of the semester. In this way, we can determine the learning gain during the course and obtain a quantitative feedback on the course. The exam is based on the learning goals of the individual chapters and the questions in the formative assessments.
|Lecture notes||The learning material and slides of the input lectures are available on Moodle. There you will also find further information (articles, links, videos).|
|Literature||All texts and references will be available on Moodle. To follow the most recent developments in this rapidly evolving field follow the following experts on Twitter:|
|Prerequisites / Notice||The course builds on the course Bio IA, in particular on that course's content regarding genetics and genomics. The course is based on self-learning units on Moodle, input lectures by experts from D-BIOL and exercises.|