Alvar Diego Gossert: Katalogdaten im Frühjahrssemester 2021

NameHerr Dr. Alvar Diego Gossert
Dep. Biologie
ETH Zürich, HPP L 25.1
Hönggerbergring 64
8093 Zürich
Telefon+41 44 633 34 55

551-0434-00LNMR Spectroscopy in Biology Belegung eingeschränkt - Details anzeigen
Number of participants limited to 6.

The enrolment is done by the D-BIOL study administration.

General safety regulations for all block courses:
-Whenever possible the distance rules have to be respected
-All students have to wear masks throughout the course. Please keep reserve masks ready. Surgical masks (IIR) or medical grade masks (FFP2) without a valve are permitted. Community masks (fabric masks) are not allowed.
-The installation and activation of the Swiss Covid-App is highly encouraged
-Any additional rules for individual courses have to be respected
-Students showing any COVID-19 symptoms are not allowed to enter ETH buildings and have to inform the course responsible
6 KP7PF. Allain, A. D. Gossert, K. Wüthrich
KurzbeschreibungIn this block course, students actively participate in ongoing research projects in the research groups of Profs. Allain, Wüthrich and Dr. Gossert. The students will be tutored in their experimental work by experienced postdoc students. In addition, the course includes specific lectures that provide the theoretical background for the experimental work, as well as exercises and literature work.
LernzielThe course provides first "hands on" insight into applications of NMR spectroscopy in biological sciences. The course should enable the students to understand the potential and limitations of NMR applied to biological problems.
InhaltThe topics include studies of proteins, RNA and protein-RNA interactions,

Participation in one of the following projects will be possible:
- NMR of RNA
- NMR of several protein-RNA complexes (hnRNPF, nPTB, SR proteins)
- NMR studies of protein-ligand interactions
- dynamics of protein-RNA complexes
- Segmental isotopic labeling to study multidomain proteins
- NMR Methods Development
SkriptNo script
LiteraturLists of individual reading assignments will be handed out.
551-1414-00LMolecular and Structural Biology V: Studying Macromolecules by NMR and EPR4 KP2VF. Allain, A. D. Gossert, G. Jeschke, K. Wüthrich
KurzbeschreibungThe course provides an overview of experimental methods for studying function and structure of macromolecules at atomic resolution in solution. The two main methods used are Nuclear Magnetic Resonance (NMR) spectroscopy and Electron Paramagnetic Resonance (EPR) spectroscopy.
LernzielInsight into the methodology, areas of application and limitations of these two methods for studying biological macromolecules. Practical exercises with spectra to have hands on understanding of the methodology.
InhaltPart I: Historical overview of structural biology.
Part II: Basic concepts of NMR and initial examples of applications.
2D NMR and isotope labeling for studying protein function and molecular interactions at atomic level.
Studies of dynamic processes of proteins in solution.
Approaches to study large particles.
Methods for determination of protein structures in solution.
Part III: NMR methods for structurally characterizing RNA and protein-RNA complexes.
Part IV: EPR of biomolecules
Literatur1) Wüthrich, K. NMR of Proteins and Nucleic Acids, Wiley-Interscience.
2) Dominguez et al, Prog Nucl Magn Reson Spectrosc. 2011 Feb;58(1-2):1-61.
3) Duss O et al, Methods Enzymol. 2015;558:279-331.
551-1616-00LMethods for Studies of Biological Macromolecules by NMR1 KP2SA. D. Gossert
KurzbeschreibungIn this course topics relevant to structure determination of biological macromolecules by solution state NMR spectroscopy are discussed. The course is tailored to advanced students, mainly PhD students and postdocs in structural biology who have experience with applications of NMR spectroscopy. The individual participants present various topics in form of a seminar.
LernzielThe students will actively participate in the course which is held in the form of a seminar. Individual students will prepare particular topics of the course based on literature references and present the material in form of a seminar to their fellow students. In short, the students learn to actively participate in discussions and to prepare a presentation of a scientific topic which was mostly unknown to them before.