701-1425-01L  Genetic Diversity: Techniques

SemesterAutumn Semester 2016
LecturersA. M. Minder Pfyl
Periodicityyearly recurring course
Language of instructionEnglish
CommentNumber of participants limited to 8.
Selection of the students: order of registration

Registration until 17.10.2016.

AbstractThis course provides training for advanced students (master, doctoral or post-doctoral level) in how to measure and collect genetic diversity data from populations, experiments, field and laboratory. Different DNA/RNA extraction, genotyping and gene expression techniques will be addressed. Choice of topic by demand and/or availability of data.
ObjectiveTo learn and improve on standard and modern methods of genetic data collection. Examples are: use of pyrosequencing, expression analysis, SNP-typing, next-generation sequencing, etc.
A course for practicioners.
ContentAfter an introduction (one afternoon), students will have 3 weeks to work independently or in groups through different protocols. At the end the whole group meets for another afternoon to present the techniques/results and to discuss the advantages and disadvantages of the different techniques.
Techniques adressed are: RNA/DNA extractions and quality control, SNP genotyping, pyrosequencing, real-time qPCR.
Lecture notesMaterial will be handed out in the course.
LiteratureMaterial will be handed out in the course.
Prerequisites / NoticeTwo afternoons are hold in the class. The lab work will be done from the students according to their timetable, but has to be finished after 3 weeks. Effort is roughly 1-2 days per week, depending on the skills of the student.