701-1425-01L Genetic Diversity: Techniques
|Semester||Autumn Semester 2016|
|Lecturers||A. M. Minder Pfyl|
|Periodicity||yearly recurring course|
|Language of instruction||English|
|Comment||Number of participants limited to 8.|
Selection of the students: order of registration
Registration until 17.10.2016.
|Abstract||This course provides training for advanced students (master, doctoral or post-doctoral level) in how to measure and collect genetic diversity data from populations, experiments, field and laboratory. Different DNA/RNA extraction, genotyping and gene expression techniques will be addressed. Choice of topic by demand and/or availability of data.|
|Objective||To learn and improve on standard and modern methods of genetic data collection. Examples are: use of pyrosequencing, expression analysis, SNP-typing, next-generation sequencing, etc. |
A course for practicioners.
|Content||After an introduction (one afternoon), students will have 3 weeks to work independently or in groups through different protocols. At the end the whole group meets for another afternoon to present the techniques/results and to discuss the advantages and disadvantages of the different techniques.|
Techniques adressed are: RNA/DNA extractions and quality control, SNP genotyping, pyrosequencing, real-time qPCR.
|Lecture notes||Material will be handed out in the course.|
|Literature||Material will be handed out in the course.|
|Prerequisites / Notice||Two afternoons are hold in the class. The lab work will be done from the students according to their timetable, but has to be finished after 3 weeks. Effort is roughly 1-2 days per week, depending on the skills of the student.|