This laboratory course has a focus on current research topics in our laboratory related to metabolic engineering, the general understanding of metabolism, and is focused particularly on C1-metabolism. Projects will be conducted in small groups.
The course aims at introducing key principles of metabolic engineering and techniques applied in metabolism related research. The main focus of this block course is on practical work and will familiarize participants with complementary approaches, in particular genetic, biochemical and analytical techniques. Results will be presented by students in scientific presentations.
The course and will include topics such as pathway elucidation & engineering and related ongoing research projects in the lab. Experimental work applied during the course will comprise methods such as cloning work & transformation, growth determination, enzyme activity assays, liquid-chromatography mass-spectrometry and dynamic labeling experiments.