535-0310-00L Glycobiology in Drug Development
|Semester||Autumn Semester 2016|
|Lecturers||V. I. Otto|
|Periodicity||yearly recurring course|
|Language of instruction||English|
|Abstract||Protein-based drugs constitute around 25% of new approvals and most of them are glycoproteins. Using selected examples the course aims at providing insight into our present knowledge on glycosylation-activity relationships and the production and analysis of glycoprotein-based drugs.|
|Objective||Gaining insight into the glycobiology of therapeutically used glycoproteins. This implies knowing and understanding|
- the major types of protein-linked glycans and their biosynthesis
- the most important expression systems for production of recombinant glycoproteins
- methods used to alter or manipulate glycosylation
- the most prominent clinically used glycoproteins and how glycosylation influences their therapeutic profile.
- Current methods for the qualitative and quantitative characterization of glycoproteins
and being able to apply this knowledge in other contexts.
1. Introduction: Carbohydrates - "life's first language"
2. Tissue plasminogen activator (t-PA), glucocerebrosidase and the biosynthesis of N-glycans
3. PSGL-1 and the biosynthesis of O-glycans;
P-selectin and other lectins
4. The glycoprotein hormones and the production and analysis of therapeutic glycoproteins
5. Monoclonal antibodies and the modification of their therapeutic profile through glycoengineering
6. EPO "the same but different"
|Lecture notes||The slides used for the lectures will be provided online|
|Literature||- Essentials of Glycobiology 2nd edition, A. Varki, R.D. Cummings et al., Cold Spring Harbor Laboratory Press, New York 2009.|
- Posttranslational Modification of Protein Biopharmaceuticals, G. Walsh (ed.), Wiley VCH, Weinheim 2009.
- Gentechnik, Biotechnik. Grundlagen und Wirkstoffe, 2. Auflage, Dingermann, Winckler, Zündorf, Wissenschaftliche Verlagsgesellschaft Stuttgart, 2011.
|Prerequisites / Notice||Requirements: Basic knowledge in immunology, molecular biology, protein chemistry and analytics. Basic knowledge in pharmacology.|