535-0310-00L Glycobiology in Drug Development
Semester | Herbstsemester 2017 |
Dozierende | V. I. Otto |
Periodizität | jährlich wiederkehrende Veranstaltung |
Lehrsprache | Englisch |
Kurzbeschreibung | Protein-based drugs constitute around 25% of new approvals and most of them are glycoproteins. Using selected examples of prominent glycoprotein drugs, the course aims at providing insight into glycosylation-activity relationships and into biotechnological production and analytics. |
Lernziel | Students gain insight into the glycobiology of therapeutically used glycoproteins. This implies knowing and understanding - the major types of protein-linked glycans and their biosynthesis - the most important expression systems for production of recombinant glycoproteins - methods used to alter or manipulate glycosylation - the most prominent clinically used glycoproteins and how glycosylation influences their therapeutic profile. - Current methods for the qualitative and quantitative characterization of glycoproteins Students are able to apply this knowledge and propose solutions to biotechnological problems that involve protein glycosylation. |
Inhalt | lecture plan: 1. Introduction: Carbohydrates - one of life's principal molecular languages 2. Glucocerebrosidase and the biosynthesis of N-glycans 3. PSGL-1 and the biosynthesis of O-glycans 4. The glycoprotein hormones and the analysis of their glycosylation 5. Monoclonal antibodies and the modification of their therapeutic profile through glycoengineering 6. EPO "the same but different" |
Skript | The slides used for the lectures will be provided online |
Literatur | - Essentials of Glycobiology 2nd edition, A. Varki, R.D. Cummings et al., Cold Spring Harbor Laboratory Press, New York 2009. - Posttranslational Modification of Protein Biopharmaceuticals, G. Walsh (ed.), Wiley VCH, Weinheim 2009. - Gentechnik, Biotechnik. Grundlagen und Wirkstoffe, 2. Auflage, Dingermann, Winckler, Zündorf, Wissenschaftliche Verlagsgesellschaft Stuttgart, 2011. |
Voraussetzungen / Besonderes | Requirements: Basic knowledge in immunology, molecular biology, protein chemistry and analytics. Basic knowledge in pharmacology. |