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535-0310-00L  Glycobiology in Drug Development

SemesterHerbstsemester 2017
DozierendeV. I. Otto
Periodizitätjährlich wiederkehrende Veranstaltung

KurzbeschreibungProtein-based drugs constitute around 25% of new approvals and most of them are glycoproteins. Using selected examples of prominent glycoprotein drugs, the course aims at providing insight into glycosylation-activity relationships and into biotechnological production and analytics.
LernzielStudents gain insight into the glycobiology of therapeutically used glycoproteins. This implies knowing and understanding
- the major types of protein-linked glycans and their biosynthesis
- the most important expression systems for production of recombinant glycoproteins
- methods used to alter or manipulate glycosylation
- the most prominent clinically used glycoproteins and how glycosylation influences their therapeutic profile.
- Current methods for the qualitative and quantitative characterization of glycoproteins
Students are able to apply this knowledge and propose solutions to biotechnological problems that involve protein glycosylation.
Inhaltlecture plan:
1. Introduction: Carbohydrates - one of life's principal molecular languages
2. Glucocerebrosidase and the biosynthesis of N-glycans
3. PSGL-1 and the biosynthesis of O-glycans
4. The glycoprotein hormones and the analysis of their glycosylation
5. Monoclonal antibodies and the modification of their therapeutic profile through glycoengineering
6. EPO "the same but different"
SkriptThe slides used for the lectures will be provided online
Literatur- Essentials of Glycobiology 2nd edition, A. Varki, R.D. Cummings et al., Cold Spring Harbor Laboratory Press, New York 2009.
- Posttranslational Modification of Protein Biopharmaceuticals, G. Walsh (ed.), Wiley VCH, Weinheim 2009.
- Gentechnik, Biotechnik. Grundlagen und Wirkstoffe, 2. Auflage, Dingermann, Winckler, Zündorf, Wissenschaftliche Verlagsgesellschaft Stuttgart, 2011.
Voraussetzungen / BesonderesRequirements: Basic knowledge in immunology, molecular biology, protein chemistry and analytics. Basic knowledge in pharmacology.