535-0310-00L Glycobiology in Drug Development
|Dozierende||V. I. Otto|
|Periodizität||jährlich wiederkehrende Veranstaltung|
|Kurzbeschreibung||Protein-based drugs constitute around 25% of new approvals and most of them are glycoproteins. Using selected examples of prominent glycoprotein drugs, the course aims at providing insight into glycosylation-activity relationships and into biotechnological production and analytics.|
|Lernziel||Students gain insight into the glycobiology of therapeutically used glycoproteins. This implies knowing and understanding|
- the major types of protein-linked glycans and their biosynthesis
- the most important expression systems for production of recombinant glycoproteins
- methods used to alter or manipulate glycosylation
- the most prominent clinically used glycoproteins and how glycosylation influences their therapeutic profile.
- Current methods for the qualitative and quantitative characterization of glycoproteins
Students are able to apply this knowledge and propose solutions to biotechnological problems that involve protein glycosylation.
1. Introduction: Carbohydrates - one of life's principal molecular languages
2. Glucocerebrosidase and the biosynthesis of N-glycans
3. PSGL-1 and the biosynthesis of O-glycans
4. The glycoprotein hormones and the analysis of their glycosylation
5. Monoclonal antibodies and the modification of their therapeutic profile through glycoengineering
6. EPO "the same but different"
|Skript||The slides used for the lectures will be provided online|
|Literatur||- Essentials of Glycobiology 2nd edition, A. Varki, R.D. Cummings et al., Cold Spring Harbor Laboratory Press, New York 2009.|
- Posttranslational Modification of Protein Biopharmaceuticals, G. Walsh (ed.), Wiley VCH, Weinheim 2009.
- Gentechnik, Biotechnik. Grundlagen und Wirkstoffe, 2. Auflage, Dingermann, Winckler, Zündorf, Wissenschaftliche Verlagsgesellschaft Stuttgart, 2011.
|Voraussetzungen / Besonderes||Requirements: Basic knowledge in immunology, molecular biology, protein chemistry and analytics. Basic knowledge in pharmacology.|