511-1003-00L  Gene Technology (Crash Course)

SemesterHerbstsemester 2017
DozierendeJ. Scheuermann
Periodizitätjährlich wiederkehrende Veranstaltung
KommentarObligatory course if assigned by the Admission committee.

KurzbeschreibungThe course enables the student to understand and and apply the general concepts of gene technology, including recombinant DNA technology and its application in genomics, transcriptomics and proteomics. Protein cloning, expression and modifications and bimolecular interactions will be discussed. The concept of display technology and its applications in the field of drug discovery will be presented.
LernzielThe students remember and understand:
1. The tools of recombinant DNA technology
2. Next generation sequencing approaches and their relevance for -omics projects
3. Protein cloning, expression, modification/labelling and oligomerization
4. Thermodynamic and kinetic affinity constants in bimolecular reactions
5. Basic structure of the antibody molecule
6. Concepts of antibody phage technology and antibody engineering
7. Construction of antibody-, peptide- or small molecule libraries and affintiy-based selection methodologies
InhaltI) Genomics:
recombinant DNA technology
methods to sequence genomes
application to human biology
Transcriptomics / Proteomics
II) Proteins:
protein cloning and expression
homo- and heterodimerization
chemical modifications and radioactive labelling
detection of bimolecular interactions
affinity constant and experimental measurement
kinetic association and dissociation constants
III) Display technology:
the antibody molecule, CDRs, basics of antibody engineering
antibody phage display and selection methodologies
construction of antibody libraries
other display technologies (peptide display, DNA-encoded chemical libraries)
Skriptslides and script used for the course and literature for reading and discussions will be made available online.
Literaturdedicated chapters of:
S.B. Primrose and R.M. Twyman 'Principles of Gene Manipulation and Genomics', 7th ed. (2006) Blackwell Science
Voraussetzungen / Besonderesadmission to MSc in Pharmaceutical Sciences