Autumn Semester 2020 takes place in a mixed form of online and classroom teaching.
Please read the published information on the individual courses carefully.

535-0810-00L  Gene Technology

SemesterAutumn Semester 2018
LecturersD. Neri
Periodicityyearly recurring course
Language of instructionEnglish

AbstractThe course will provide a solid overview of the science and issues in gene technology and its pharmaceutical applications.
ObjectiveThe aim of the lecture course is to provide a solid overview of gene technology, with a special focus on drug development. Topics: Antibody phage technology, DNA-encoded chemistry, protein modification technology, genome sequencing, transcriptomics, proteomics, functional genomics, principle of drug discovery. The course is suited for advanced undergraduate and early graduate students in pharmaceutical sciences or related fields.
Content1. Antibody phage technology
The antibody molecule
V genes, CDRs, basics of antibody engineering
Principles of phage display
Phagemid and phage vectors
Antibody libraries
Phage display selection methodologies
Other phage libraries (peptides, globular proteins, enzymes)
Alternative screening/selection methodologies
DNA-encoded chemical libraries

2. Proteins: chemical modification and detection of biomolecular interactions
Homo- and hetero-dimerization of proteins
Chemical modifications of proteins
Antibody-drug conjugates
Radioactive labeling of proteins
Kinetic association and dissociation constants
Affinity constant: definition and its experimental measurement

3. Genomics: Applications to Human Biology
Protein cloning and expression
DNA sequencing
Some foundations of genetic analysis
Knock-out technologies
Recombinant vaccines

4: Pharmaceuticals: Focus on Discovery
Ligand Discovery
Half-life extension
Cancer therapy
Gene therapy
Lecture notesSkript "Gene Technology" by Prof. Dario Neri and slides of the lecture