From 2 November 2020, the autumn semester 2020 will take place online. Exceptions: Courses that can only be carried out with on-site presence.
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535-0310-00L  Glycobiology in Drug Development

SemesterAutumn Semester 2018
LecturersV. I. Otto
Periodicityyearly recurring course
Language of instructionEnglish

AbstractProtein-based drugs constitute around 25% of new approvals and most of them are glycoproteins. Using selected examples of prominent glycoprotein drugs, the course aims at providing insight into glycosylation-activity relationships and into biotechnological production and analytics.
ObjectiveStudents gain insight into the glycobiology of therapeutically used glycoproteins. This implies knowing and understanding
- the major types of protein-linked glycans and their biosynthesis
- the most important expression systems for production of recombinant glycoproteins
- methods used to alter or manipulate glycosylation
- the most prominent clinically used glycoproteins and how glycosylation influences their therapeutic profile.
- Current methods for the qualitative and quantitative characterization of glycoproteins
Students are able to apply this knowledge and propose solutions to biotechnological problems that involve protein glycosylation.
Contentlecture plan:
1. Glycans - information carriers in biology and pharmacotherapy
2. Glucocerebrosidase and the biosynthesis of N-glycans
3. Glyocerebrosidase - production and quality control
4. Improving the therapeutic profile of monoclonal antibodies by glycoengineering
5. Sialylation and mucin-type O-glycans as critical quality attributes of glycoprotein hormones and drugs
6. EPO "the same but different"
The lectures will include some exercises in which students apply their knowledge to solve simple biotechnological problems related to protein glycosylation.
Lecture notesThe slides used for the lectures will be provided online
Literature- Essentials of Glycobiology 3rd edition, A. Varki, R.D. Cummings et al., Cold Spring Harbor Laboratory Press, New York 2017.
- Posttranslational Modification of Protein Biopharmaceuticals, G. Walsh (ed.), Wiley VCH, Weinheim 2009.
- Gentechnik, Biotechnik. Grundlagen und Wirkstoffe, 2. Auflage, Dingermann, Winckler, Zündorf, Wissenschaftliche Verlagsgesellschaft Stuttgart, 2011.
Prerequisites / NoticeRequirements: Basic knowledge in immunology, molecular biology, protein chemistry and analytics. Basic knowledge in pharmacology.