551-1556-00L  X-Ray Crystallographic Structure Determination and Biophysics

SemesterSpring Semester 2016
LecturersK. Locher, G. Schertler, D. Veprintsev
Periodicityyearly recurring course
Language of instructionEnglish
CommentNumber of participants limited to 11 in the 3rd semester quarter of the spring semester
Number of participants limited to 12 in the 4th semester quarter of the spring semester



Courses

NumberTitleHoursLecturers
551-1556-00 GX-Ray Crystallographic Structure Determination and Biophysics
Permission from lecturers required for all students.
Block course will be held two times, once in the 3rd- and once in the 4th semester quarter of the spring semester
The practical part of the course for 4 students in each quarter will be done at the Laboratory of Biolmolecular Research (BMR), Paul Scherrer Institut (Link). For location please see the link Link
100s hrs
Tue12:45-16:30HPK D 15 »
Wed07:45-16:30HPK D 15 »
Thu07:45-16:30HPK D 15 »
Fri07:45-16:30HPK D 15 »
K. Locher, G. Schertler, D. Veprintsev

Catalogue data

AbstractThis course will familiarize the students with techniques used for the biophysical and structural characterization of proteins. The students will carry out biophysical characterization of the proteins with dynamic light scattering and CD spectroscopy.
ObjectiveThe course aims at introducing the principles of protein X-ray crystallography as well as other techniques used in the biophysical characterization of proteins. Students will get an opportunity to conduct hands-on experiments and also use computational techniques.
ContentMicro-calorimetry and fluorescence anisotropy measurements will be used to study protein peptide interactions. The course also includes a demonstration of the Synchrotron capabilities at the Paul Scherrer Institute (SLS). Students will crystallize a protein, collect X-ray diffraction data using an in-house X-ray source, and build an atomic model into the electron density map.
Prerequisites / NoticeThe students will be both at ETH Honggerberg and the Paul Scherrer Institute (PSI) at Villigen. Transport will be organized by rental car or by public transportation.

Performance assessment

Performance assessment information (valid until the course unit is held again)
Performance assessment as a semester course
ECTS credits6 credits
ExaminersK. Locher, G. Schertler, D. Veprintsev
Typegraded semester performance
Language of examinationEnglish
RepetitionRepetition only possible after re-enrolling for the course unit.

Learning materials

No public learning materials available.
Only public learning materials are listed.

Groups

No information on groups available.

Restrictions

GeneralPermission from lecturers required for all students
Beginning of registration periodRegistration possible from 08.02.2016

Offered in

ProgrammeSectionType
Biology BachelorBlock Courses in 3rd Quarter of the SemesterWInformation
Biology BachelorBlock Courses in 4th Quarter of the SemesterWInformation