636-0201-00L  Lab Course: Methods in Cell Analysis and Laboratory Automation

SemesterHerbstsemester 2017
DozierendeT. Horn
Periodizitätjährlich wiederkehrende Veranstaltung
LehrspracheEnglisch
KommentarOnly for Biotechnology MSc, Programme Regulations 2017.


KurzbeschreibungThe course Methods in Cell Analysis and Laboratory Automation introduces students to high-end cell analysis and sample preparation methods including image analysis. Students will be taught theoretical aspects and skills in Flow Cytometry, Light Microscopy, Image Analysis, and the use of Laboratory Automation.
Lernziel-to understand the technical and physical principles of light microscopes and flow cytometers
-to have hands-on experience in the use of these technologies to analyze/image real samples
-to be able to run a basic analysis of the data and images obtained with flow cytometers and microscopes
-to get introduced to liquid handling (pipetting) robotics and learn how to implement a basic workflow
InhaltThe practical course will have five units at 2 days each (total 10 days):
1. Flow Cytometry:
a. Introduction to Flow Cytometry
b. Practical demonstration on flow cytometry analyzers and flow cytometry cell sorters
c. Flow cytometry sample preparation
d. Learn how to use flow cytometry equipment to analyze and sort fluorescence-labeled cells
2. Light microscopy
a. Learn how to build a microscope and understand the underlying physical principles
b. Learn how to use a modern automated wide field fluorescence microscope
c. Use this microscope to automatically acquire images of a cell culture assay to analyze the dose-dependent effect of a drug treatment
3. Image Analysis
a. Introduction to the fundamentals of image analysis
b. Learn the basics of the image analysis software Fiji/ImageJ
c. Use Fiji/ImageJ to analyze the images acquired during the microscopy exercise
4. Laboratory Automation
a. Introduction to the basics of automated liquid handling/ lab robotics
b. See examples on using lab automation for plasmid library generation and cell cultivation
c. Learn how to program and execute a basic pipetting workflow including liquid handling and labware transfers on Tecan and Hamilton robotic systems
5. Presentations
a. Each student will be assigned to an individual topic of the course and will have to prepare a presentation on it.
b. Presentations and discussion in form of a Colloquium
SkriptYou will find further information on the practical course and the equipment at:
https://www.bsse.ethz.ch/scf
https://www.bsse.ethz.ch/laf
LiteraturMicroscopy: Murphy and Davidson, Fundamentals of Light Microscopy and Electronic Imaging, John Wiley & Sons, 2012
Flow Cytometry: Shapiro, Practical Flow Cytometry, John Wiley & Sons, 2005
Image analysis: R. C. Gonzalez, R. E. Woods, Digital Image Processing (3rd Edition), Prentice Hall
Laboratory Automation: Design and construction of a first-generation high-throughput integrated robotic molecular biology platform for bioenergy applications (2011) J. Lab. Autom., 16(4), 292-307
Voraussetzungen / BesonderesThe following knowledge is required for the course:
-basic laboratory methods
-basic physics of optics (properties of light, refraction, lenses, fluorescence)
-basic biology of cells (cell anatomy and physiology)