752-0400-00L  Microscopy

SemesterSpring Semester 2019
LecturersG. H. Dasen
Periodicityyearly recurring course
Language of instructionGerman
CommentNumber of participants limited to 50.


AbstractThis course focuses on teaching the students the modern methods of food analysis by light microscopy. We will analyse and discuss various components present in food. Examples of the investigated products are yoghurt, cheese, dried mushrooms and honey.
In addition, the theoretical background of light microscopy will be discussed.
ObjectiveThe Students know the range of application of light microscopy for the analysis of food products according to national and international guidelines (e.g. ISO).
They can select and combine suitable preparation, contrasting and colouring procedures as well as use digital documentation techniques.
The microscopists can reset a completely misaligned microscope to its optimal working condition.
In their professional life the students should be able to oversee the acquisition of microscopes and supplies.
ContentKöhler illumination
Image formation according to Abbe
Fundamentals of optical contrasting methods (phase contrast, dark field, polarisation, fluorescence)
Microorganisms in food (bacteria, yeast, moulds)
Staining techniques in food microbiology (e.g. Gram, spores, pianese)
Staining techniques for food components (starch, lipids, proteins)
Sample preparation and documentation (incl. digital microphotography)
Quantitative microscopy (size and number)
Applications in food microscopy (filth, pollen in honey)
Lecture notesFood Microscopy (G. Dasen)
Literaturesee German version
Prerequisites / NoticeMaterial: light- and stereo-microscopes

max. number of students per course: 20