551-0352-00L  Protein Analysis by Mass Spectrometry

SemesterSpring Semester 2019
LecturersL. Gillet, B. Collins, P. Picotti
Periodicityyearly recurring course
Language of instructionEnglish
CommentNumber of participants limited to 12.

The enrolment is done by the D-BIOL study administration.

AbstractProtein Analysis by Mass Spectrometry
The following topics will be covered: basics of biological mass spectrometry, including instrumentation, data collection and data analysis; applications to protein identification and characterization; sample preparation methods; proteomics strategies; and quantitative analysis.
ObjectiveHow to prepare a protein sample for MS analysis (trypsin digestion, C18 clean-up)
Principles of data acquisition LC-MS (QTOF and/or Ion Trap instruments)
Perform qualitative proteomic analysis (protein identification with Mascot and/or Sequest Softwares)
Perform quantitative proteomic analysis (label-free and labeled analyses)
Analyze/interpret the data to find up/down regulated proteins