551-0352-00L Protein Analysis by Mass Spectrometry
| Semester | Spring Semester 2019 |
| Lecturers | L. Gillet, B. Collins, P. Picotti |
| Periodicity | yearly recurring course |
| Language of instruction | English |
| Comment | Number of participants limited to 12. The enrolment is done by the D-BIOL study administration. |
| Abstract | Protein Analysis by Mass Spectrometry The following topics will be covered: basics of biological mass spectrometry, including instrumentation, data collection and data analysis; applications to protein identification and characterization; sample preparation methods; proteomics strategies; and quantitative analysis. |
| Objective | How to prepare a protein sample for MS analysis (trypsin digestion, C18 clean-up) Principles of data acquisition LC-MS (QTOF and/or Ion Trap instruments) Perform qualitative proteomic analysis (protein identification with Mascot and/or Sequest Softwares) Perform quantitative proteomic analysis (label-free and labeled analyses) Analyze/interpret the data to find up/down regulated proteins |