551-1700-00L  Introduction to Flow Cytometry

SemesterSpring Semester 2021
LecturersJ. Kisielow, L. Tortola, further lecturers
Periodicityyearly recurring course
Language of instructionEnglish
CommentNumber of participants limited to 24.



Courses

NumberTitleHoursLecturers
551-1700-00 VIntroduction to Flow Cytometry1 hrs
Tue08:45-09:30HPL D 32 »
J. Kisielow, L. Tortola, further lecturers

Catalogue data

AbstractThe lecture provides an introduction to flow cytometry. We will cover the technology basics, experimental design, data acquisition and analysis of flow and mass cytometry. In addition, various research applications will be discussed. The format is a lecture course enriched by a visit to the ETH Flow Cytometry Core Facility and practical demonstration of the use of analysis and sorting instruments.
ObjectiveThe goal of this course is to provide the basic knowledge of flow and mass cytometry required for planning and execution of cytometric experiments.
ContentThe lecture course aims at teaching principles of flow cytometry. The emphasis is on theoretical principles (signal detection, fluorochromes, signal spill-over and compensation) as well as practical aspects of experimental design and performance (sample preparation, controls, data acquisition and analysis).
List of topics:
- Principles of Flow Cytometry
- Signal processing
- Compensation and Controls
- Data analysis, gating and presentation
- Panel design
- Sorting
- Mass cytometry
- High-dimensional data analysis
- Practical demonstration (hardware and software)
Modern flow cytometric techniques for immunophenotyping, analysis of proliferation, cell cycle, apoptosis and cell signalling will be introduced.
Lecture notesUpdated handouts will be provided during the class.
LiteratureCurrent literature references on immunophenotyping, analysis of proliferation, cell cycle, apoptosis and cell signalling will be discussed during the lectures.

Performance assessment

Performance assessment information (valid until the course unit is held again)
Performance assessment as a semester course
ECTS credits2 credits
ExaminersJ. Kisielow
Typesession examination
Language of examinationEnglish
RepetitionThe performance assessment is offered every session. Repetition possible without re-enrolling for the course unit.
Mode of examinationwritten 60 minutes
Additional information on mode of examinationOnly the main examiner is listed above.
Written aidsNone
This information can be updated until the beginning of the semester; information on the examination timetable is binding.

Learning materials

No public learning materials available.
Only public learning materials are listed.

Groups

No information on groups available.

Restrictions

Places24 at the most
Waiting listuntil 15.02.2021

Offered in

ProgrammeSectionType
Biology MasterElective Compulsory Master CoursesWInformation
Biology MasterElective Compulsory Master CoursesWInformation
Biology MasterElective Compulsory Master CoursesWInformation
Doctoral Department BiologyDoctoral and Post-Doctoral CoursesWInformation