551-1403-00L  Imaging Bacterial Cells in a Native State by Electron Cryotomography

SemesterAutumn Semester 2015
LecturersM. Pilhofer
Periodicityyearly recurring course
Language of instructionEnglish
CommentNumber of participants limited to 3.



Courses

NumberTitleHoursLecturers
551-1403-00 GImaging Bacterial Cells in a Native State by Electron Cryotomography
Permission from lecturers required for all students.
Block course in the 4th quarter of the autumn semester
Number of participants limited to 3.
100s hrs
Tue12:45-16:30HPK D 6.1 »
Wed07:45-16:30HPK D 6.1 »
Thu07:45-16:30HPK D 6.1 »
Fri07:45-16:30HPK D 6.1 »
M. Pilhofer

Catalogue data

AbstractThe goal is to acquire the techniques to image bacteria by electron cryotomography, resolving their structure in a native state, in 3D, and to macromolecular resolution. In a small group, students will perform wet lab experiments, data collection with stat-of-the-art equipment, data processing and analyses. The key method and its application in bacterial cell biology will be introduced by lectures
ObjectiveStudents will acquire the skills to cultivate bacteria, plunge-freeze samples for cryotomography, collect data using an electron cryomicroscope, process raw data, analyze tomograms, perform subtomogram averaging, model structures of interest, and generate movies for visualization.
Link

Performance assessment

Performance assessment information (valid until the course unit is held again)
Performance assessment as a semester course
ECTS credits6 credits
ExaminersM. Pilhofer
Typegraded semester performance
Language of examinationEnglish
RepetitionRepetition only possible after re-enrolling for the course unit.

Learning materials

No public learning materials available.
Only public learning materials are listed.

Groups

No information on groups available.

Restrictions

GeneralPermission from lecturers required for all students
Beginning of registration periodRegistration possible from 31.08.2015

Offered in

ProgrammeSectionType
Biology BachelorBlock Courses in 4th Quarter of the SemesterWInformation