551-0352-00L Introduction to Mass Spectrometry-based Proteomics
|Semester||Spring Semester 2020|
|Lecturers||L. Gillet, P. Picotti|
|Periodicity||yearly recurring course|
|Language of instruction||English|
|Comment||Number of participants limited to 12.|
The enrolment is done by the D-BIOL study administration.
|Abstract||Protein Analysis by Mass Spectrometry|
The following topics will be covered: basics of biological mass spectrometry, including instrumentation, data collection and data analysis; applications to protein identification and characterization; sample preparation methods; proteomics strategies; and quantitative analysis.
|Objective||How to prepare a protein sample for MS analysis (trypsin digestion, C18 clean-up)|
Principles of data acquisition LC-MS (QTOF and/or Ion Trap instruments)
Perform qualitative proteomic analysis (protein identification with Mascot and/or Sequest Softwares)
Perform quantitative proteomic analysis (label-free and labeled analyses)
Analyze/interpret the data to find up/down regulated proteins