551-0307-01L  Biomolecular Structure and Mechanism II: Large Cellular Machines

Semester Spring Semester 2015
Lecturers N. Ban, F. Allain, T. Ishikawa, M. Pilhofer
Periodicity yearly course
Language of instruction English

Abstract This Concept Course will cover advanced topics in Molecular Biology with particular emphasis on the structure and function of large cellular assemblies.
Objective This concept course will further develop general topics introduced in basic biochemistry classes (for example: Fundamentals of Biologie II: Biochemistry and Molecular Biology - 551-1323-00L) with emphasis on the architecture and the function of large cellular machines involved in DNA replication, transcription, translation, nucleic acid packaging in viruses, RNA processing, function of large multienzymes, motor proteins and injection assemblies.
Specifically the following topics will be covered:
1) Translation, advanced topics,
2) Co-translational protein processing, folding and targeting to the membranes,
3) Virus structure principles,
4) Multienzyme function and architecture,
5) DNA replication, Chromatin structure, remodeling,
6) Injection machines, structure and mechanism,
7) ATP-driven motor proteins,
8) Transcription, prokaryotic and eukaryotic, transcription factors -DNA specific vs non specific recognition,
9) Principles of RNA structures
10) Ribozymes and self-splicing
11) RNA processing machineries Capping, Splicing, 3'end processing and polyadelylation
12) Splicing regulation
Content Advanced class covering the state of the research in structural molecular biology of basic cellular processes with emphasis on the function of large cellular assemblies.
Lecture notes Updated handouts will be provided during the class.
Literature The lecture will be based on the latest literature. Additional suggested
Branden, C., and J. Tooze, Introduction to Protein Structure, 2nd ed.
(1995). Garland, New York.