551-1403-00L  Imaging Bacterial Cells in a Native State by Electron Cryotomography

SemesterAutumn Semester 2015
LecturersM. Pilhofer
Periodicityyearly course
Language of instructionEnglish
CommentNumber of participants limited to 3.

AbstractThe goal is to acquire the techniques to image bacteria by electron cryotomography, resolving their structure in a native state, in 3D, and to macromolecular resolution. In a small group, students will perform wet lab experiments, data collection with stat-of-the-art equipment, data processing and analyses. The key method and its application in bacterial cell biology will be introduced by lectures
ObjectiveStudents will acquire the skills to cultivate bacteria, plunge-freeze samples for cryotomography, collect data using an electron cryomicroscope, process raw data, analyze tomograms, perform subtomogram averaging, model structures of interest, and generate movies for visualization.