551-1556-00L  X-Ray Crystallographic Structure Determination and Biophysics

SemesterSpring Semester 2016
LecturersK. Locher, G. Schertler, D. Veprintsev
Periodicityyearly course
Language of instructionEnglish
CommentNumber of participants limited to 11 in the 3rd semester quarter of the spring semester
Number of participants limited to 12 in the 4th semester quarter of the spring semester

AbstractThis course will familiarize the students with techniques used for the biophysical and structural characterization of proteins. The students will carry out biophysical characterization of the proteins with dynamic light scattering and CD spectroscopy.
ObjectiveThe course aims at introducing the principles of protein X-ray crystallography as well as other techniques used in the biophysical characterization of proteins. Students will get an opportunity to conduct hands-on experiments and also use computational techniques.
ContentMicro-calorimetry and fluorescence anisotropy measurements will be used to study protein peptide interactions. The course also includes a demonstration of the Synchrotron capabilities at the Paul Scherrer Institute (SLS). Students will crystallize a protein, collect X-ray diffraction data using an in-house X-ray source, and build an atomic model into the electron density map.
Prerequisites / NoticeThe students will be both at ETH Honggerberg and the Paul Scherrer Institute (PSI) at Villigen. Transport will be organized by rental car or by public transportation.