551-0142-00L  Structure Determination of Biological Macromolecules by X-ray Crystallography, EM and NMR

SemesterSpring Semester 2016
LecturersF. Allain, N. Ban, K. Locher, G. Wider, K. Wüthrich, further lecturers
Periodicityyearly course
Language of instructionEnglish

AbstractThe course provides an overview of experimental methods for the determination of structures of macromolecules at atomic resolution.
ObjectiveInsight into the methodology, areas of application and limitations of two principal methods for the structure determination of biological macromolecules.
ContentPart I: Methods for the determination of the structure of proteins and macromolecular complexes using X-ray diffraction in single crystals.
Part II: Methods for the determination of protein structures in solution using nuclear magnetic resonance (NMR) spectroscopy. Experimental approaches to the characterization of intramolecular dynamics of proteins.
Literature1) Wüthrich, K. NMR of Proteins and Nucleic Acids, Wiley-Interscience.
2) Blow, D. Outline of Crystallography for Biologists. Oxford University Press.