551-0142-00L Structure Determination of Biological Macromolecules by X-ray Crystallography, EM and NMR
|Semester||Spring Semester 2016|
|Lecturers||F. Allain, N. Ban, K. Locher, G. Wider, K. Wüthrich, further lecturers|
|Language of instruction||English|
|Abstract||The course provides an overview of experimental methods for the determination of structures of macromolecules at atomic resolution.|
|Objective||Insight into the methodology, areas of application and limitations of two principal methods for the structure determination of biological macromolecules.|
|Content||Part I: Methods for the determination of the structure of proteins and macromolecular complexes using X-ray diffraction in single crystals. |
Part II: Methods for the determination of protein structures in solution using nuclear magnetic resonance (NMR) spectroscopy. Experimental approaches to the characterization of intramolecular dynamics of proteins.
|Literature||1) Wüthrich, K. NMR of Proteins and Nucleic Acids, Wiley-Interscience. |
2) Blow, D. Outline of Crystallography for Biologists. Oxford University Press.