551-0142-00L  Structure Determination of Biological Macromolecules by X-ray Crystallography, EM and NMR

Semester Spring Semester 2016
Lecturers F. Allain, N. Ban, K. Locher, G. Wider, K. Wüthrich, further lecturers
Periodicity yearly course
Language of instruction English


Abstract The course provides an overview of experimental methods for the determination of structures of macromolecules at atomic resolution.
Objective Insight into the methodology, areas of application and limitations of two principal methods for the structure determination of biological macromolecules.
Content Part I: Methods for the determination of the structure of proteins and macromolecular complexes using X-ray diffraction in single crystals.
Part II: Methods for the determination of protein structures in solution using nuclear magnetic resonance (NMR) spectroscopy. Experimental approaches to the characterization of intramolecular dynamics of proteins.
Literature 1) Wüthrich, K. NMR of Proteins and Nucleic Acids, Wiley-Interscience.
2) Blow, D. Outline of Crystallography for Biologists. Oxford University Press.
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