Tatjana Kleele: Catalogue data in Autumn Semester 2024 |
| Name | Prof. Dr. Tatjana Kleele |
| Field | Mitochondrial Biology |
| Address | Institut für Biochemie ETH Zürich, HPM E 17.2 Otto-Stern-Weg 3 8093 Zürich SWITZERLAND |
| Telephone | +41 44 633 43 12 |
| tatjana.kleele@bc.biol.ethz.ch | |
| Department | Biology |
| Relationship | Assistant Professor |
| Number | Title | ECTS | Hours | Lecturers | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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| 551-0319-00L | Cellular Biochemistry (Part I) | 3 credits | 2V | U. Kutay, F. Allain, T. Kleele, I. Zemp | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Abstract | Concepts and molecular mechanisms underlying the biochemistry of the cell, providing advanced insights into structure, function and regulation of individual cell components. Particular emphasis will be put on the spatial and temporal integration of different molecules and signaling pathways into global cellular processes such as intracellular transport, cell division & growth, and cell migration. | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Learning objective | The full-year course (551-0319-00 & 551-0320-00) focuses on the molecular mechanisms and concepts underlying the biochemistry of cellular physiology, investigating how these processes are integrated to carry out highly coordinated cellular functions. The molecular characterisation of complex cellular functions requires a combination of approaches such as biochemistry, but also cell biology and genetics. This course is therefore the occasion to discuss these techniques and their integration in modern cellular biochemistry. The students will be able to describe the structural and functional details of individual cell components, and the spatial and temporal regulation of their interactions. In particular, they will learn to explain the integration of different molecules and signaling pathways into complex and highly dynamic cellular processes such as intracellular transport, cytoskeletal rearrangements, cell motility, cell division and cell growth. In addition, they will be able to illustrate the relevance of particular signaling pathways for cellular pathologies such as cancer. | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Content | Structural and functional details of individual cell components, regulation of their interactions, and various aspects of the regulation and compartmentalisation of biochemical processes. Topics include: biophysical and electrical properties of membranes; viral membranes; structural and functional insights into intracellular transport and targeting; vesicular trafficking and phagocytosis; post-transcriptional regulation of gene expression. | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Lecture notes | Scripts and additional material will be provided during the semester. Please contact Dr. Alicia Smith for assistance with the learning materials. (alicia.smith@bc.biol.ethz.ch) | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Literature | Recommended supplementary literature (review articles and selected primary literature) will be provided during the course. | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Prerequisites / Notice | To attend this course the students must have a solid basic knowledge in chemistry, biochemistry and general biology. The course will be taught in English. | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Competencies |
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| 551-0336-00L | Methods in Cellular Biochemistry  Number of participants limited to 19. The enrolment is done by the D-BIOL study administration. | 6 credits | 7P | I. Zemp, T. Kleele, V. Korkhov, U. Kutay, T. Michaels, M. Peter | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Abstract | Students will learn about biochemical approaches to analyze cellular functions. The course consists of practical projects in small groups, lectures and literature discussions. The course concludes with the presentation of results at a poster session. | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Learning objective | Students will learn to design, carry out and assess experiments using current biochemical and cell biological strategies to analyze cellular functions in model systems. In particular they will learn novel imaging techniques along with biochemical approaches to understand fundamental cellular pathways. Furthermore, they will learn to assess strengths and limitations of the different approaches and be able to discuss their validity for the analysis of cellular functions. | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Literature | Documentation and recommended literature (review articles and selected primary literature) will be provided during the course. | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Prerequisites / Notice | This course will be taught in English. | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Competencies |
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| 551-0357-00L | Cellular Matters: Properties, Functions and Applications of Biomolecular Condensates The number of participants is limited to 30 and will only take place with a minimum of 6 participants. At the beginning of the course, student groups will be formed and assigned to the milestone papers. To facilitate this, students must confirm their registration by the beginning of the 3rd week of semester. | 4 credits | 2S | T. Michaels, F. Allain, P. Arosio, D. Hilvert, M. Jagannathan, T. Kleele, R. Mezzenga, G. Neurohr, R. Riek, A. E. Smith, K. Weis, further lecturers | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Abstract | This Master level course delves into the emerging field of biomolecular condensates - membrane-less organelles in cells. Using interdisciplinary concepts from biology, chemistry, biophysics, and soft matter, we will explore the biological properties of these condensates, their functions in health and disease, and their potentiol as new biomimetic materials for various applications. | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Learning objective | In the last decade, a novel type of cell compartments called biomolecular condensates have been discovered. This discovery is radically changing our understanding of the cell, its organization, and dynamics. The emerging picture is that the cytoplasm and nucleoplasm are highly complex fluids that can (meta)stably segregate into membrane-less compartments, similary to emulsions. This interdisciplinary course encompasses milestone works and cutting-edge research questions in the young field of biomolecular condensates, including their properties, functions, and applications. The course begins with a lecture series that introduces the topic of condensates to an interdisciplinary audience and provides a theoretical foundation for understanding current research questions in the field. the lecturesprovide a base for student presentations of recent publications in the field, and for research seminars given by course lecturers, who are all active researchers with diverse expertise. Through this exciting interdisciplinary understanding of biomolecular condensates, bridging biology, chemistry, biophysics, and soft matter. Students will not only learn how to critically read and evaluate scientific literature but will also gain valuable experience in giving scientific presentations to an interdisciplinary audience. Each presentation will require an introduction, critical analysis of the results, and a discussion of their significance, allowing student to substantiate their statements with a critical mindset that considers the pros and cons of chosen approaches and methods, as well as any limitations or possible follow-up experiments. This process will enable student to ask relevant querions and actively participate in class discussions, further enhancing their scientific skills. In preparing the presentations, the students will have the unique opportunity to interact closely with each other and with the lecturers, who are all internationally well-established experts, and receive guidance in selectin a topic for the final presentaton and supporting literature. | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Content | The topic of biomolecular condensates goes beyond the boundaries of traditional disciplines and requires a multi-disciplinary approach that leverages and cross-fertilizes biology, physical chemistry, biophysics, and soft matter. This course will explore the properties, functions and potentioal applicatons of biomolecular condensates, including their role in neurodegenerative diseases such as Alzheimer's and Parkinson's, as well as their use as smart biomimetic materials. This course is divided into two parts. The fist part will introduce the basic concepts essentialto the study of biomolecular condensates and phase separation. Topics include: fundamental units and scales in soft matter, phase transitions in biology, biopolymers and molecular self-assembly, introduction to active matter. This will establish a foundation for the second part, which will explore milestone works and current research in the field of biomolecular condensates. Each lecture of this second part will consist of: 1) a short literature seminar, where student groups will present and discuss a milestone paper assigned in advance and 2) a research seminar, where one of the course lecturers will present their own state-of-the art research in the field, building upon the milestone literature. At the beginning of the course, student groups will be formed and assigned to the milestone papers. To facilitate this, students must confirm their registration by the beginning of the 3rd week of semester. | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Lecture notes | Lecture slides and some scripts will be provided. | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Literature | No compulsory textbooks. Literature will be provided during the course | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Competencies |
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| 551-1303-00L | Current Research Topics in Cellular Biochemistry | 4 credits | 2S | T. Kleele, V. Korkhov, G. Neurohr, V. Panse, M. Peter, A. E. Smith, F. van Drogen | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Abstract | During this Masters level seminar style course, students will explore current research topics in cellular biochemistry focused on the structure, function and regulation of selected cell components, and the consequences of dysregulation for pathologies. | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Learning objective | Students will work with experts toward a critical analysis of cutting-edge research in the domain of cellular biochemistry. At the end of the course, students will be able to evaluate, critically discuss and write about scientific articles in the research area of cellular biochemistry, and they will identify open testable research questions. | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Content | Guided by an expert in the field, students will engage in classical round-table style discussions of current literature with frontal presentations. Guided by a faculty expert, students develop domain knowledge of the research area associated with a primary paper, and then develop a mini-review document. It is possible to align the course focus to an onging or future research project in the domain of the course tutors. At the end of the semester, students present as a group to an overview of the reasearch domain and research perspectives. | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Literature | The literature will be provided during the course. | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Prerequisites / Notice | The course will be taught in English. | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Competencies |
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