701-1425-01L Genetic Diversity: Techniques
| Semester | Autumn Semester 2021 |
| Lecturers | A. M. Minder Pfyl |
| Periodicity | yearly recurring course |
| Language of instruction | English |
| Comment | Number of participants limited to 8. Waiting list will be deleted November 1st, 2021. No enrollment possible after October 18th, 2021. |
| Abstract | This course provides training for advanced students (master, doctoral or post-doctoral level) in how to measure and collect genetic diversity data from populations, experiments, field and laboratory. Different DNA/RNA extraction protocols, quality control measurements, SNP genotyping and gene expression techniques will be addressed. |
| Learning objective | To learn and improve on standard and modern methods of genetic data collection. Examples are: use of pyrosequencing, expression analysis, SNP-typing, next-generation sequencing etc. A course for practicioners. |
| Content | After an introduction (one afternoon), students will have 3 weeks to work independently in groups of two through different protocols. At the end the whole class meets for another afternoon to present the techniques/results and to discuss the advantages and disadvantages of the different techniques. Techniques adressed are: RNA/DNA extractions and quality control, SNP genotyping, pyrosequencing, real-time qPCR. |
| Lecture notes | Material will be handed out in the course. |
| Literature | Material will be handed out in the course. |
| Prerequisites / Notice | Two afternoons are hold in the class. The lab work will be done from the students according to their timetable, but has to be finished after 3 weeks. Effort is roughly 1-2 days per week, depending on the skills of the student. |