Start of the course: Wednesday, 3.11.21 at 13:15-17:00, end of the course: Wednesday, 24.11.21 at 13:15-17:00, individual work in between (about one whole day per week preferably Monday to Wednesday). Course room for the introduction and final discussion will be announced, lab work has to be done at the GDC.
This course provides training for advanced students (master, doctoral or post-doctoral level) in how to measure and collect genetic diversity data from populations, experiments, field and laboratory. Different DNA/RNA extraction protocols, quality control measurements, SNP genotyping and gene expression techniques will be addressed.
Learning objective
To learn and improve on standard and modern methods of genetic data collection. Examples are: use of pyrosequencing, expression analysis, SNP-typing, next-generation sequencing etc. A course for practicioners.
Content
After an introduction (one afternoon), students will have 3 weeks to work independently in groups of two through different protocols. At the end the whole class meets for another afternoon to present the techniques/results and to discuss the advantages and disadvantages of the different techniques. Techniques adressed are: RNA/DNA extractions and quality control, SNP genotyping, pyrosequencing, real-time qPCR.
Lecture notes
Material will be handed out in the course.
Literature
Material will be handed out in the course.
Prerequisites / Notice
Two afternoons are hold in the class. The lab work will be done from the students according to their timetable, but has to be finished after 3 weeks. Effort is roughly 1-2 days per week, depending on the skills of the student.
Performance assessment
Performance assessment information (valid until the course unit is held again)
Performance assessment as a semester course