Prerequisties: Participation in the course Introduction to Nutritional Science (752-6001-00) and Introduction to Toxicology (752-1300-00) in parallel to the laboratory course.
Courses
Number
Title
Hours
Lecturers
752-6210-00 P
Laborpraktikum Toxikologie und Ernährung
Permission from lecturers required for all students.
Das Praktikum wird teils auf Deutsch und teils auf Englisch unterrichtet.
Das Praktikum findet im Zeitraum vom 25.04.2023 bis 19.05.2023 statt. Das detaillierte Programm wird separat bekannt gegeben. Das Praktikum ist mit den Exkursionen II abgestimmt.
Introduction to the analysis of pesticide residues in food, learning to assess cytotoxicity of a chemical in mammalian cells, monitoring the stability of DNA by UV spectrophotometry and synthesizing an antioxidant. Introduction to anthropometric measurements and their interpretation, dietary assessment including analysis using nutritional software, assessment and interpretation of iron status.
Learning objective
1) To gain practical insights into food monitoring by applying an LC-MS screening method and legal requirements for pesticides. 2) To learn details about mammalian cell cultures and practical training on the use of methods to measure cell viability. 3) To study the properties of a DNA duplex by melting the native structure while monitoring the transition with UV spectrophotometry. 4) To preform an organic chemistry reaction and recrystallization of an antioxidant and use UV spectrophotometry to assess the antioxidant capacity of structurally similar compounds. 5) To learn to assess and interpret anthropometric measurements, such as assessment of growth, fat-free mass and body fat 6) To gain insight into the concept of dietary assessment and to use one of the methods in an exercise and analyze and interpret the data using nutritional software 7) To understand the complexity of iron status measurement and the factors influencing its interpretation.
Content
1) To gain practical insights into food monitoring by applying an LC-MS screening method and legal requirements for pesticides. 2) To learn details about mammalian cell cultures and practical training on the use of methods to measure cell viability. 3) To study the properties of a DNA duplex by melting the native structure while monitoring the transition with UV spectrophotometry. 4) To preform an organic chemistry reaction and recrystallization of an antioxidant and use UV spectrophotometry to assess the antioxidant capacity of structurally similar compounds. 5) To learn to assess and interpret anthropometric measurements, such as assessment of growth, fat-free mass and body fat 6) To gain insight into the concept of dietary assessment and to use one of the methods in an exercise and analyze and interpret the data using nutritional software 7) To understand the complexity of iron status measurement and the factors influencing its interpretation.
Lecture notes
Complete course documenation will be provided.
Literature
References will be indicated in the lab course material.
Competencies
Subject-specific Competencies
Concepts and Theories
assessed
Techniques and Technologies
assessed
Method-specific Competencies
Analytical Competencies
assessed
Project Management
fostered
Social Competencies
Cooperation and Teamwork
fostered
Performance assessment
Performance assessment information (valid until the course unit is held again)