Overview of modern chemical biology and synthetic biochemistry techniques, focussed on protein modification and labeling and on methods to endow proteins with novel functionalities.
Learning objective
After taking this course, students should be capable of the following: A) Recall different possibilities for modifying proteins in vitro and in vivo and their applications in a biological context, B) Understand the chemical and biochemical consequences of modifications and assess the different reaction possibilities in the context of in vivo - in vitro, C) Critically analyze and assess current chemical biology articles D) Question the approaches learned and apply them to new biological problems.
Content
principles of protein labeling and protein modification (fluorescent proteins, enzyme-mediated labeling, bioorthogonal chemistries)
advanced genetic code expansion methods (amber suppression, orthogonal ribosomes, unnatural base pairs, genome engineering and genome editing)
directed evolution and protein engineering
chemical biology of ubiquitin and targeted protein degradation
Lecture notes
A script will not be handed out. Handouts to the lecture will be provided through moodle.
Literature
Citations from the original literature relevant to the individual lectures will be assigned during the lectures.
Prerequisites / Notice
Knowledge provided in the bachelor lectures 'Nucleic Acids and Carbohydrates' and 'Proteins and Lipids' is assumed for this lecture.
Competencies
Subject-specific Competencies
Concepts and Theories
assessed
Techniques and Technologies
assessed
Method-specific Competencies
Analytical Competencies
assessed
Decision-making
fostered
Media and Digital Technologies
fostered
Problem-solving
assessed
Project Management
fostered
Social Competencies
Communication
fostered
Cooperation and Teamwork
fostered
Customer Orientation
fostered
Leadership and Responsibility
fostered
Self-presentation and Social Influence
fostered
Sensitivity to Diversity
fostered
Negotiation
fostered
Personal Competencies
Adaptability and Flexibility
fostered
Creative Thinking
assessed
Critical Thinking
assessed
Integrity and Work Ethics
fostered
Self-awareness and Self-reflection
fostered
Self-direction and Self-management
fostered
Performance assessment
Performance assessment information (valid until the course unit is held again)
Performance assessment as a semester course