551-0352-00L  Introduction to Mass Spectrometry-Based Proteomics

SemesterAutumn Semester 2023
LecturersL. Gillet, P. Picotti
Periodicityyearly recurring course
Language of instructionEnglish
CommentNumber of participants limited to 12.

The enrolment is done by the D-BIOL study administration.


AbstractProtein Analysis by Mass Spectrometry
The following topics will be covered: basics of biological mass spectrometry, including instrumentation, data collection and data analysis; applications to protein identification and characterization; sample preparation methods; proteomics strategies; and quantitative analysis.
Learning objectiveHow to prepare a protein sample for MS analysis (trypsin digestion, C18 clean-up)
Principles of data acquisition LC-MS (QTOF and/or Ion Trap instruments)
Perform qualitative proteomic analysis (protein identification with Mascot and/or Sequest Softwares)
Perform quantitative proteomic analysis (label-free and labeled analyses)
Analyze/interpret the data to find up/down regulated proteins
CompetenciesCompetencies
Subject-specific CompetenciesConcepts and Theoriesassessed
Techniques and Technologiesassessed
Method-specific CompetenciesAnalytical Competenciesassessed
Decision-makingassessed
Problem-solvingassessed
Social CompetenciesCommunicationassessed
Cooperation and Teamworkfostered
Personal CompetenciesCreative Thinkingfostered
Critical Thinkingfostered