529-0733-02L Chemical Biology and Synthetic Biochemistry
| Semester | Autumn Semester 2023 |
| Lecturers | K. Lang, M. Fottner |
| Periodicity | yearly recurring course |
| Language of instruction | English |
| Abstract | Overview of modern chemical biology and synthetic biochemistry techniques, focussed on protein modification and labeling and on methods to endow proteins with novel functionalities. | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Learning objective | After taking this course, students should be capable of the following: A) Recall different possibilities for modifying proteins in vitro and in vivo and their applications in a biological context, B) Understand the chemical and biochemical consequences of modifications and assess the different reaction possibilities in the context of in vivo - in vitro, C) Critically analyze and assess current chemical biology articles D) Question the approaches learned and apply them to new biological problems. | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Content | principles of protein labeling and protein modification (fluorescent proteins, enzyme-mediated labeling, bioorthogonal chemistries) advanced genetic code expansion methods (amber suppression, orthogonal ribosomes, unnatural base pairs, genome engineering and genome editing) directed evolution and protein engineering chemical biology of ubiquitin and targeted protein degradation | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Lecture notes | A script will not be handed out. Handouts to the lecture will be provided through moodle. | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Literature | Citations from the original literature relevant to the individual lectures will be assigned during the lectures. | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Prerequisites / Notice | Knowledge provided in the bachelor lectures 'Nucleic Acids and Carbohydrates' and 'Proteins and Lipids' is assumed for this lecture. | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Competencies |
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